Hybridization of RZPD-161c1 and RZPD-1070i12 full clone probes againstNotI–EcoRI digests of clones mapping on regions 15q26.1 (left) and 15q24 (right), respectively. For RZPD-161c1 probe: lane 1, BAC RZPD-1017c8 (15q26.1); lane2, BAC RZPD-415o6 (15q26.1); lane 3, BAC RZPD-1070i12 (15q26.1); lane 4, BAC RZPD-416g15 (15q26.1, negative control); and lane 5, BAC RZPD-161c1 (15q24, positive control). Clone RZPD-416g15 constitutes the negative control as it overlaps at the opposite end of the REP471 sequence (see Fig. 1). Clone RZPD-1017c8 does not hybridize with the two larger low copy repeat fragments (**), further confirming the distal orientation of this repeated sequence from REP471 sequence. For probe RZPD-1070i12: lane1, BAC RG-471g13 (15q26.1, positive control); lane 2, BAC RZPD-215f12 (BAC clone mapped on 15q24 that does not show amplification with REP471 sequence; negative control); lane 3, BAC RZPD-161c1 (15q24). Hybridization fragments that constitute the two LCR15s are marked by asterisks. The positive fragments add up to 20 kb for RZPD-161c1 and 26 kb for RZPD-1070i12, defining the estimated sizes of the LCR15 at 15q26.1 and 15q24, respectively (to the 20 kb we have to add 2 kb detected with RG-471g13; not shown). An arrow marks the fragment that corresponds to the vector hybridization.
