(A) Genomic contig of the 15q25.3–q26.1 region. The radiation hybrid map corresponds to the Genebridge 4 panel (Whitehead Institute for Biomedical Research/MIT Center for Genome Research), from 334 cR to 341 cR (1 cR ∼240 kb). STSs L802b4, SHGC-34665 and REP471 are included in rectangles. Genes are shown in red and UniGene clusters in blue. UniGene clusters derived from pDJ clones (10k5, 105i19, and 68d5) are oriented in relation to the 5′ or 3′ ends of genesPOLG, CRALBP, and IQGAP1, respectively. Filled and unfilled circles correspond to the presence or absence of markers, respectively. Clones shown in yellow were mapped by FISH on 15q25.3–q26.1; clones in green rectangles were mapped on 15q11–q13 and 15q25.3–q26.1; and clones in red rectangles were mapped on 6q12–q13. An arrow marks clone RZPD-1070i12 (LCR15–1) shown in Figure4. HGMP, UK Human Genome Mapping Project Resource Centre (RPCI-1 PAC library); RG, Research Genetics (CITB BAC library); and RZPD, Resource Center within the German Human Genome Project (RPCI-11 BAC library). Only the largest BAC/PAC clones and clones mentioned in the text are shown; information about other positive clones for markers of this region is available from the authors. The relative position of the gap within the contig is marked by a filled triangle. A group of clones positive for SHGC-34665 but probably not mapping to 15q25.3–q26.1 is shown in brackets. PFGE NotI restriction fragments detected with different probes are shown as bidirectional arrows. The relative location of the low copy repeats on 15q26.1 (LCR15–1.a/b/c) is shown as an orange box. The LCR15–1.b and LCR15–1.c are located according to the marker content of the respective RPCI-11 clones (groups 7 and 8 in Fig. 5C; D15S901for clone 286b10; WI-14285 and D15S642 for clones 341b7 and 95f11, respectively). The markers and regions containing sequence similarities with other chromosomes or chromosome 15q regions are shown. The location of the centromere (CEN) and telomere (TEL), the region involved in the congentital fibrosarcoma (CFS) translocation and the orientation of Bloom's disease (BLM) gene are indicated. (B) Partial genomic contig of chromosome 15q24 and identification of low copy repeat sequences. Clones within boxes were positive for REP471 (LCR15) and are grouped in three nonoverlapping blocks (a, b, and c). An arrow marks clone RZPD-161c1 shown in Figure4. The relative location of LCR15–2 (a, b, and c) on 15q24 are shown. (Figure continues on following page.)
