Requirements for fs(1)Yb (A-F ) and brn(G-I ) in patterning of the germarium. fcs, somatic follicle cells; gc, germ cells; R1: germarial region 1. (A) Actin staining of fs(1)Yb72 /fs(1)Yb72 mutant germarium revealing multiple cysts within a single egg chamber. Arrows point to oocytes. Scale bar = 50μm. (B,C) Sxl localization in 1-d-old wild type (B) and fs(1)Yb72 /fs(1)Yb72 (C) mutant germaria. Scale bar = 10μm. (D,E,F) Adducin-like localization in 1-d-old wild type (D), 1-d-old fs(1)Yb72 /fs(1)Yb72 (E), and 6-d–oldfs(1)Yb72 /fs(1)Yb72 (F) germaria. Arrow in (D) points to a branched fusome. Arrows in (E,F) point to unbranched fusomes (spectrosomes). Scale bars = 10μm. (G) Actin staining of a brn198 /brn198 mutant ovariole which lacks stalk cells, resulting in failure to separate egg chambers. Three egg chambers are labeled with arrowheads. (H) Germline staining with anti-Vasa antibody of a wild type germarium and early-stage egg chambers, revealing the continuously growing germline cells. (I) Vasa staining of abrn228 /brn228 ovariole revealing depletion of germline cells. Scale bar in (H,I) = 50μm.
