New genes required for patterning of the germarium. gl, germline; fcs, follicle cells. (A) Adducin-like localization infs(1)124/fs(1)124 mutant germarium reveals spectrosome-containing cells and small cysts with branched fusomes. (B) Nuclear staining of anfs(1)259/fs(1)259 egg chamber containing four polyploid nurse cells and one oocyte nucleus (arrow). Scale bar = 10μm. (C) Actin staining of anfs(1)259/fs(1)259 egg chamber containing multiple nurse cell/oocyte cysts. Arrows point to oocytes. (D) Nuclear staining of a late stage 9 wild type egg chamber. (E) Nuclear staining of a late stage 9fs(1)217/fs(1)217 egg chamber from a 2-d-old female, revealing a reduced number of follicle cells. Scale bar = 50μm. (F) Large fused egg chamber from a 6-d-oldfs(1)217/fs(1)217 female. Only a small number of follicle cells surround this egg chamber. Arrows point to follicle cell nuclei.
