Pyrosequencing Sheds Light on DNA Sequencing

Table 1.

Kinetic Data of Enzymes Involved in Pyrosequencing

Enzyme K M(μM) k cat (S−1)
Klenow Polymerase 0.18 (dTTP) 0.92
ATP sulfurylase 0.56 (APS) 7.0 (PPi) 38
Firefly luciferase 20 (ATP) 0.015
Apyrase 120 (ATP) 260 (ADP) 500  (ATP)

  • ↵ Van Draanen et al. 1992.

  • ↵ Nyren and Lundin 1985.

  • ↵ DeLuca and McElroy 1984.

  • ↵ Traverso-Cori et al. 1965.

This Article

  1. Genome Res. 11: 3-11

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