Pyrosequencing Sheds Light on DNA Sequencing

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 6.
Figure 6.

Pyrogram obtained from five different chambers using microfluidics in a spinning CD-format device developed at Gyros AB (Sweden). All chambers contained the same DNA template. The addition order of nucleotides was GCTA. Nucleotides G, T, and G were correctly incorporated. Background signals for nucleotides C and A were due to pyrophosphate (PPi) contamination in the pyrosequencing reaction mixture.

This Article

  1. Genome Res. 11: 3-11

Preprint Server