Gene Expression Changes Triggered by Exposure of Haemophilus influenzae to Novobiocin or Ciprofloxacin: Combined Transcription and Translation Analysis

Table 1.

Reproducibility of the mRNA Quantification

Sample preparation Experiment NF Increased or decreased Fold change >2 or <–2
Same culture, 1 0.98 1 0
 same RNA preparation, 2 1.27 29 6
 independent labeling and 3 0.66 7 0
 hybridization
Same culture, 1 0.88 32 7
 independent RNA 2 1.12 3 1
 preparation, labeling, and 3 1.34 13 1
 hybridization 4 1.70 3 0
Different cultures 1.22 3.33 61
  • (NF) Normalization factor.

  • Transcripts called increased or decreased according to the Affymetrix GeneChip software.

  • Fold changes between −2.1 and −2.5.

  • Genes total = 1961.

This Article

  1. Genome Res. 11: 28-42

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