High-Throughput SNP Genotyping by Allele-Specific PCR with Universal Energy-Transfer-Labeled Primers

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 4.
Figure 4.

Fluorescence intensity measurements for four SNP assays. The 21 samples of genomic DNA (40 ng per reaction) were analyzed for an A/T SNP (RS363), a C/T SNP (CYP17), a C/G SNP (HTR2C), and a G/T SNP (MAOA). Each plate also contained three controls without DNA. The red and green fluorescence intensity was determined in a Victor II plate reader (Wallac).

This Article

  1. Genome Res. 11: 163-169

Preprint Server