Robust and Accurate Single Nucleotide Polymorphism Genotyping by Dynamic Allele-Specific Hybridization (DASH): Design Criteria and Assay Validation

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Figure 5.
Figure 5.

An example of incorrect genotype assignment by DASH. The curve representing the incorrectly assigned genotype is shown both in and by the arrow. A weak aberrant high-temperature peak gave the impression of a heterozygote, when, in fact, the sample was a homozygote, which should have produced a single low-temperature peak. Three confirmed genotypes (both homozygotes, and one heterozygote) are included for comparison.

This Article

  1. Genome Res. 11: 152-162

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