Figure 3.
Redesign details are shown for five DASH assays. In each case, primer sequences (5′—3′) are indicated for the original nonfunctional assay and for the repaired functional assay, along with the respective theoretically predicted (mfold) secondary structures of the resulting PCR product. The leftmost secondary structure represents the original design, and the rightmost structure represents the situation after primer sequence modification. Sequences in which a 5′-biotin moiety has been added are marked with the letter b. SNP accession numbers according to HGBASE (http://hgbase.cgr.ki.se) are shown for each assay.
