Table 1.
Mitotic Stability of the HCV
| Cell line | Population doublings | Loss rate | +G418 | −G418 | |||||
| 0 | 1 | 2 | 0 | 1 | 2 | ||||
| E10B1 | 109 | 0.23% | 1 (1%) | 107 (95%) | 4 (4%) | 19 (14%) | 103 (78%) | 10 (8%) | |
| ES clone E | 40 | 2.66% | 2 (4%) | 48 (96%) | 0 | 33 (66%) | 17 (34%) | 0 | |
| ES clone F | 40 | 1.03% | 0 | 50 (100%) | 0 | 17 (34%) | 33 (66%) | 0 | |
| ES clone G | 40 | 0.26% | 3 (6%) | 47 (94%) | 0 | 5 (10%) | 45 (90%) | 0 | |
| ES clone H | 40 | 0.62% | 1 (2%) | 49 (98%) | 0 | 11 (22%) | 39 (78%) | 0 | |
| ES clone I | 40 | 0.82% | 1 (2%) | 49 (98%) | 0 | 14 (28%) | 36 (72%) | 0 | |
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The cell lines were cultured in the presence or absence of G418. The HCVs were detected by FISH with a human alphoid-2 probe. The number of metaphase spreads showing respectively 0, 1 and 2 HCVs are given (percentages in brackets).
