Table 1.
Primers Used for Heteroduplex Analysis
| Position | Primers | Ta | Tm | Size |
| 185delAG | Forward: 5′GAAGTTGTCATTTTATAAACCTTT-3′ | 53 | 56 | 258 bp |
| (Exon 2, BRCA1) | Reverse: 5′-TGTCTTTTCTTCCCTAGTATGT-3′ | 53 | ||
| Forward 1: 5′-TCCAACACTTGTTATTTGGT-3′ | 53 | 56 | 159 bp | |
| E1250X | Reverse 1: 5′-CCTTTGCCAATATTACCTG-3′ | 53 | ||
| (Exon 11,BRCA1) | Forward 2: 5′-TTTCACCCATACACATTTG-3′ | 53 | 56 | 260 bp |
| Reverse 1: 5′-CCTTTGCCAATATTACCTG-3′ | 53 | |||
| R1443G | Forward: 5′-AGCTGTGTTAGAACAGCATG-3′ | 54 | 56 | 204 bp |
| (Exon 13,BRCA1) | Reverse: 5′-TGTTGGAGCTAGGTCCTTAC-3′ | 54 | ||
| Forward 1: 5′-ATATGACGTGTCTGCTCCAC-3′ | 56 | 58 | 257 bp | |
| Reverse 1: 5′-AGTCTTACAAAATGAAGCGG-3′ | 55 | |||
| 5382insC | Forward 1: 5′-ATATGACGTGTCTGCTCCAC-3′ | 56 | 58 | 296 bp |
| (Exon 20,BRCA1) | Reverse 2: 5′-CCTGTGTGAAAGTATCTAGCAC-3′ | 54 | ||
| Forward 1: 5′-ATATGACGTGTCTGCTCCAC-3′ | 56 | 58 | 399 bp | |
| Reverse 3: 5′-GGGAATCCAAATTACACAGC-3′ | 57 | |||
| Forward 1: 5′-GTTGGACAGTGTAGCACTCTA-3′ | 53 | 56 | 136 bp | |
| 5677insA | Reverse 1: 5′-CCACTTTGTAAGCTCATTCTT-3′ | 54 | ||
| (Exon 24,BRCA1) | Forward 2: 5′-ATGAATTGACACTAATCTCTGC-3′ | 54 | 56 | 223 bp |
| Reverse 1: 5′-CCACTTTGTAAGCTCATTCTT-3′ | 54 | |||
| 6174delT | Forward: 5′-CACCTTGTGATGTTAGTTTGGA-3′ | 58 | 58 | 201 bp |
| (Exon 11,BRCA2) | Reverse: 5′-TGGAAAAGACTTGCTTGGTACT-3′ | 58 |
