Figure 7.
Detection of the homozygous mutation and two heterozygous mutations by capillary electrophoresis–based heteroduplex analysis. (A) wild type (wt); (B) HCC 1937 breast cancer cells (hm; containing 5382insC homozygous mutation); (C)5382insC heterozygous mutation (ht); (D) reannealing (re) PCR amplicons from wild type and HCC 1937 (each 5 μl) were mixed, heated at 95°C for 5 min, and cooled at 0°C for 5 min before injection. The separation was carried out at 20°C, other CE conditions were as in Fig. 1.
