Figure 6.
Fast mutation detection via heteroduplex analysis on a microfabricated electrophoretic chip. The heterozygous mutants were specified in the figure. The separation buffer was 2.5% HEC containing 10% glycerol in 1 × TBE (pH = 8.6) for (a–d) and 4.5% HEC containing 10% glycerol and 15% urea in 1 × TBE (pH = 8.6) for (e–f). The microchannel on the chip was coated with PVP (Mr 1,000,000) and detection mediated by laser-induced fluorescence (em/ex 520 nm/488 nm). The PCR products were injected into the channel for 100 sec at 333 V/cm (effective microchannel length of 5.5 cm), and the separation voltage was 573 V/cm.
