Rapid Detection of Deletion, Insertion, and Substitution Mutations via Heteroduplex Analysis Using Capillary- and Microchip-Based Electrophoresis

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Figure 4.
Figure 4.

Detection sensitivity with capillary electrophoresis-based heteroduplex analysis. Panels (A) and (B) show the heteroduplex analysis results with the 6174delT and 5677insAmutants. Wild-type DNA template and the mutated DNA template were mixed together and coamplified by PCR for analysis by CE-LIF. The mixed percentages of the mutated DNA template were specified in the figure. The separation buffer was 2.5% HEC containing 10% glycerol in 1 × TBE. Other CE conditions were as in Fig. 1.

This Article

  1. Genome Res. 10: 1403-1413

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