Figure 3.
Effect of sample salt concentration on capillary electrophoresis-based heteroduplex analysis. Panels (A) and (B) show the heteroduplex analysis results with the mutants,5382insC and 5677insA, respectively. The PCR products injected were: (a) PCR mixture, (b) PCR product diluted 10 times with deionized water, (c) PCR product purified by AutoSeq G-50, and (d) PCR product purified by Microcon YM-100. The separation buffer consisted of 2.5% HEC containing 10% glycerol in 1 × TBE (pH = 8.6). Other CE conditions were as in Fig. 1.
