Table 2.
Candidate Glioblastoma Tumor Markers
| Gene name (synonym) | Symbol (GenBank no.) | Cellular location | Primer pairs | Ta°C |
| Sec61 gamma | SEC61G (AF054184) | ER-Golgi (Greenfield and High 1999) | TTA CTT TAA TTT AGA AAT AG/ATC AGG TAA TGC AGT TTG TT | 50 |
| Nicotinamide N-methyltransferase | NNMT (U08021) | Cytoplasm (Aksoy et al. 1994) | CTG CCT AGA CGG TGT GAA G/AGT GGC TGG CTC TGA GTC AC | 55 |
| ATP-binding cassette, subfamily C Member 3 (MLP2, MRP3, CMOAT2) | ABCC3 (AB010887) | Membrane (Kiuchi et al. 1998) | CAT CGA CCT GGA GAC TGA CAA C/ CCA TTC TGC GGA CAT ATT TG | 58 |
| Neuromedin B (NMB) | NMB (M21551) | Secreted (Krane et al. 1988) | AGC CAG CAA GAT CCG AGT G/GCA CAA TCT AAG CCA CGC TG | 50 |
| Annexin A1 (lipocortin 1, LPC1) | ANXA1 (X05908) | Membrane (Wallner et al. 1986) | GCA GGC CTG GTT TAT TGA AA/GGT TGC TTC ATC CAC ACC TT | 53 |
| SPARC: secreted protein, acidic, cysteine-rich (osteonectin) | SPARC (J03040) | Secreted (Lane and Sage 1994) | AGG TCA CAG GTC TCG AAA A/AGA GGT GGT GGA AGA AAC TG | 53 |
| Glycoprotein (transmembrane) nmb | GPNMB (X76534) | Membrane (Weterman et al. 1995) | AAC TCT ACC CAG TGT GGA AG/TTG AGG AAG TGG CTA GGA TC | 55 |
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↵Ta refers to the annealing temperature used for fluorescent-PCR amplification.
