Identification of chromosomal breakpoints on human cosmid sequences. A schematic presentation of predicted and known genes on three cosmids near the synteny and inversion breakpoints is shown. Size and distance between exons is approximate and not to scale. The predicted gene sequences were blasted, and the inserts of IMAGE clones corresponding to identified mouse ESTs were PCR-amplified and mapped by pulsed-field gel electrophoresis (PFGE) and genetic mapping. The mouse loci names D10Bur1e and D10Bur2e are those assigned by the mouse nomenclature committee. The human predicted genes have not yet been named. The mouse Ebi3 gene was mapped genetically to mouse chromosome 17 on a publicly available panel (Rowe et al. 1994). Based on the boundary of the genes, the synteny break and the inversion break have been localized to small regions less than 5 kb in size, between the EBI3 and R33243_2, and betweenR33590_1 and SIRT6. The accession numbers for cosmids R33590, R33243, and F20887 are AC005620, AC006930, and AC005578.
