Identification of single nucleotide polymorphisms (SNPs) in biotin-labeled templates bound to streptavidin-coated paramagnetic beads (Dynabeads Streptavidin), by pyrosequencing. Primers were extended in the antisense direction. Sequencing primers and incorporated nucleotides are shown below each pyrogram series as an arrow and bold letters, respectively. Arbitrary luminescense units are shown on the ordinate axis. Each SNP is indicated by an arrowhead doublet. The two top diagrams show the respective homozygous test samples, whereas those below represent heterozygous counterparts. (A) A/T polymorphism, located in the human angiotensin I-converting enzyme promoter (A-240T). (B) C/T variation in the human angiotensin I-converting enzyme gene in exon 8 (T1237C). (C) Identical with (b), except that an allele-separating nucleotide dispensing scheme (within the biallelic variation) was applied.
