Loss of Heterozygosity Assay for Molecular Detection of Cancer Using Energy-transfer Primers and Capillary Array Electrophoresis

Table 2.

Sequences and Characteristics of ET Primers

Marker name Chromosome location Repeat PCR product size range (bps) Forward primer sequence Reverse primer sequence
IFN-A 9p23-9p22 di (gt) 130–155 TGCGCGTTAAGTTAATTGGTT M′GTAAGGT*GGAAACCCCCACT
D9S171 9p21-pp21 di (ca) 150–185 AGCTAAGTGAACCTCATCTCTGTCT M′ACCCT*AGCACTGATGGTATAGTCT
D9S162 9p22-9p21 di (ca) 160–205 M′GCAAT*GACCAGTTAAGGTTC AATTCCCACAACAAATCTCC
D9S747 9pter-9pter tetra (gata) 170–210 GCCATTATTGACTCTGGAAAAGAC M′CAGGCT*CTCAAAATATGAACAAAAT
  • (M′) Indicates the location of conjugation to the common cyanine donor dye (CYA).

  • (T*) Indicates the modified T nucleotide where the acceptor dyes, ROX or R110, were conjugated. (Hung et al. 1996; Hung et al. 1998; Ju et al. 1995)

This Article

  1. Genome Res. 10: 1211-1218

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