Comparison of Radioactive and ET-CAE Fluorescent LOH Assays
| Locus | D9S747 | D9S162 | D9S171 | IFN-A | ||||||||
| sample set | RA | FL | ratio | RA | FL | ratio | RA | FL | ratio | RA | FL | ratio |
| 1 | NI | NI | – – | LOH | .77 | – – | LOH | .68 | – – | – – | ||
| 2 | NI | NI | – – | – – | – – | – – | – – | – – | ||||
| 3 | NI | NI | – – | LOH | .69 | – – | LOH | .54 | NI | NI | ||
| 4 | NI | NI | NI | NI | – – | – – | – – | – – | ||||
| 5 | – – | – – | – – | LOH/MI | .57 | – – | – – | – – | – – | |||
| 6 | LOH | – – | .90 | – – | LOH | .37 | NI | LOH/MI | .11 | NI | NI | |
| 7 | – – | – – | – – | – – | – – | – – | – – | – – | ||||
| 8 | NI | NI | LOH | LOH | .11 | – – | LOH | .48 | NI | NI | ||
| 9 | – – | – – | NI | NI | – – | – – | – – | – – | ||||
| 10 | – – | LOH | .74 | – – | – – | – – | LOH | .78 | – – | – – | ||
| 11 | LOH | – – | .86 | NI | NI | – – | – – | – – | – – | |||
| 12 | NI | NI | – – | – – | – – | – – | – – | – – | ||||
| 13 | – – | – – | NI | NI | – – | LOH | .68 | – – | – – | |||
| 14 | – – | – – | – – | – – | – – | – – | – – | – – | ||||
| 15 | – – | – – | NI | NI | NI | NI | – – | – – | ||||
| 16 | – – | – – | LOH | LOH | .76 | – – | – – | – – | – – | |||
| 17 | – – | – – | LOH | LOH | .41 | – – | – – | – – | – – | |||
| 18 | – – | – – | – – | – – | NI | NI | – – | – – | ||||
| 19 | NI | NI | – – | – – | NI | NI | NI | NI | ||||
| 20 | – – | – – | – – | – – | – – | – – | NI | NI | ||||
| 21 | UN | UN | LOH | LOH | .75 | – – | – – | LOH | UN1 | |||
| 22 | LOH | LOH | .36 | LOH | LOH/MI | .76 | LOH | LOH | .38 | LOH | LOH | .30 |
| 23 | – – | LOH | .59 | NI | NI | LOH | LOH | .50 | – – | – – | ||
| 24 | LOH | LOH | .07 | LOH | UN | LOH | LOH | .78 | LOH | LOH | .56 | |
| 25 | – – | – – | – – | – – | – – | LOH | .77 | – – | – – | |||
| 26 | NI | LOH | .33 | LOH/MI | UN | – – | LOH | .55 | – – | – – | ||
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(RA) LOH assay via radioisotope labeling and analysis (as described in Eisenberger et al. 1999; Mao et al. 1996; Steiner et al. 1997).
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(FL) Fluorescent ET-CAE LOH assay as described in Methods.
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(– –) Indicates no LOH detected.
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Ratio: Normalized allelic ratio for fluorescent assay.
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(NI) Not informative, locus genotyped as a homozygote.
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(LOH) Indicates LOH detected by method indicated with an allelic ratio variation of 20% which is considered diagnostically significant (Wang et al. 1997).
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(MI) Microsatellite repeat instability detected.
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(UN) Unable to assay/interpret (possible PCR failure, etc.) or 1Incomplete analysis due to limited sample DNA.
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↵Unable to resolve alleles clearly on MegaBACE system.
