Loss of Heterozygosity Assay for Molecular Detection of Cancer Using Energy-transfer Primers and Capillary Array Electrophoresis

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Figure 4.
Figure 4.

Two-color electropherograms from the D9S162 loci demonstrating nucleotide repeat expansion or deletion associated with microsatellite instability (MI). (A) Ten-bp size increase caused by an expansion of five di-nucleotide repeats in the ET-ROX (red) amplified tumor sample as compared to the ET-R110 (blue) amplified normal sample (sample set 5). (B) Ten-bp size loss caused by the deletion of five di-nucleotide repeats in the ET-ROX (red) tumor sample as compared to the ET-R110 (blue) normal sample (sample set 22).

This Article

  1. Genome Res. 10: 1211-1218

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