Loss of Heterozygosity Assay for Molecular Detection of Cancer Using Energy-transfer Primers and Capillary Array Electrophoresis

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Figure 2.
Figure 2.

Two-color LOH assays using complex samples (sample set 10). (A,B) Electropherograms from a normal sample amplified at the D9S171 locus with both ET-ROX (red) and ET-R110 (blue) primers. (C,D) Electropherograms from the matching tumor samples with both primer sets. (E,F) The resulting electropherograms from co-injected two-color samples. The numbers given at the right of each panel are the allelic ratios. The arrows indicate the peaks used for allele ratio determination.

This Article

  1. Genome Res. 10: 1211-1218

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