Discovery of a Novel, Paternally Expressed Ubiquitin-specific Processing Protease Gene through Comparative Analysis of an Imprinted Region of Mouse Chromosome 7 and Human Chromosome 19q13.4

Paternal expression of mouse Usp29. PCR amplification was performed across the 19-bp insertion/deletion polymorphism located in the 3′ untranslated region of mouseUsp29. RNAs derived from the F1 hybrid (male M. spretus × female M. musculus) and the F2 hybrid (maleM. musculus × female F1) were used for this imprinting test. Three genomic DNAs—M. spretus (lane 1), M. musculus(lane 2), and F1 hybrid (lane 3)—are also included in this test. Exclusive paternal expression of mouse Usp29 is observed when using RNAs derived from embryos (lanes 4–6), neonatal (lane 7), and adult (lane 8) brains of F1 offspring, and also the neonatal brain (lane 9) of F2 animals.