Localization of Usp29 transcripts in mouse embryo and adult brain. (A) Whole mount in situ hybridization of Usp29 to 10.5-dpc embryo. Usp29 expression (stained in dark blue) was detected at high levels in the face, forebrain (fb), hindbrain (hb), forelimb (fl), and hindlimb (hl). (B) Sagittal section of whole adult mouse brain hybridized with Usp29 probe.Cells expressing the Usp29transcript are stained in red; the tissue has been counterstained with hematoxylin, which stains nuclei and therefore regions that are dense in neuronal cell bodies blue. Regions expressing Usp29at significant levels include cerebellum (cb), hippocampus (hc), habenula (hn), hypothalamus (hy), ependyma (ep), and cerebral cortex (cc). High-power magnification of selected Usp29-expressing regions, showing cell types expressing the gene. Left panel (cb): Section of the cerebellum, showing high levels ofUsp29 expression in Purkinje cells (pc) but not in the densely packed granule cells (gc); Center left panel (cc): section of the cerebral cortex, showing high levels of expression in pyramidal cells throughout the cerebral cortex; Center right panel (ep):expression in ependymal cells; and Right panel (hc): showing high levels of Usp29 expression in the granule layer of the hippocampus.
