
Pasting text sequence information into the text box and clicking on the “Submit” button (left) retrieves the tag and orientation information given below the text box. Tags are given in four possible sequence orientations, the top two orientations being the most common. The correct tag and orientation can be chosen based on the poly-adenylation signal and tail location/presence information. Clicking on the tag (left) will retrieve tag-to-gene mapping information (right) including reliable gene assignment; relative and absolute tag abundance in the SAGE libraries currently in the NCBI SAGE repository; and every UniGene identifier, type, orientation, frequency, and GenBank Accession numbers for each GenBank and dbEST sequence which contained that tag. The information in the right panel can also be retrieved by typing in the 10-base tag sequence in the search box at the top of the page. This same information for UniGene clusters can be retrieved with a similar search interface (not shown) which is located athttp://www.ncbi.nlm.nih.gov/SAGE/SAGEcid.cgi.











