Data for the 40 Clones Chosen for RT–PCR Analysis
| Clone I.D. | Computational sequence analysis predictions | RT–PCR oligo(dT) primed cDNA | Chromosome No. | RH map position/chromosome size | GenBank matches (Aug. 1999) |
| ALP5 | ✔ | 22 | 23.8/184.1 | — | |
| ALP12 | — | — | 1 | / | — |
| ALP14 | exon, CpG | — | × | / | — |
| ALP15 | ✔ | 8 | 503.9/565.8 | — | |
| ALP27 | ✔ | 16 | / | gene | |
| ALP31 | CpG | M | / | / | EST |
| ALP32 | poly(A) | ✔ | / | / | — |
| ALP33 | — | M | 10 | 499.6/556.9 | — |
| ALP35 | exon, CpG | M | / | / | gene |
| ALP44 | I | 22 | 45.3/184.1 | — | |
| ALP46 | CpG | M | × | 185.2/817.9 | — |
| ALP55 | poly(A) | M | 19 | 270.3/306.2 | — |
| ALP60 | poly(A), CpG | ✔ | 6 | 465.7/636.4 | — |
| ALP71 | exon, CpG | ✔ | 7 | 423.4/698.7 | EST |
| ALP74 | CpG | M | 5 | 341.7/664.1 | EST/gene |
| ALP79 | — | ✔ | 16 | / | — |
| ALP80 | poly(A) | ✔ | 9 | 361.4/438.0 | EST |
| ALP86 | — | I | 6 | 402.3/636.4 | — |
| ALP88 | exon, poly(A) | M* | 6 | 596.8/636.4 | — |
| ALP91 | — | M* | 18 | 20.8/489.3 | — |
| ALP96 | exon | — | 16 | 38.0/508.0 | EST |
| ALP105 | poly(A) | I | 11 | 127.9/442.8 | — |
| ALP107 | — | M* | 1 | 779.4/817.9 | EST/gene |
| ALP110 | — | I | 7 | 111.5/698.7 | — |
| ALP120 | exon | — | 22 | 45.3/184.1 | EST/gene |
| ALP121 | exon, CpG | — | 18 | 399.2/489.3 | — |
| ALP130 | — | — | 10 | 465.6/556.9 | — |
| ALP131 | exon | — | 12 | 397.6/508.4 | — |
| ALP141 | poly(A) | I | 11 | 125.9/442.8 | — |
| ALP143 | — | 5 | / | EST/gene | |
| ALP145 | — | 15 | 346.6/347.9 | — | |
| ALP148 | ✔ | 16 | 370.0/508.0 | — | |
| ALP155 | — | ✔ | 10 | 108.1/556.9 | — |
| ALP157 | exon, CpG | ✔ | / | / | — |
| ALP160 | — | — | / | / | — |
| ALP163 | exon | — | 13 | 257.2/329.7 | — |
| ALP168 | exon | M | / | / | EST/gene |
| ALP169 | ✔ | 22 | 49.5/184.1 | — | |
| ALP176 | — | 15 | 311.5/347.9 | — | |
| ALP178 | — | 1* (H) | / | — |
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(Column 1) Clone I.D. (Column 2) Computational analysis results. (Column 3) RT–PCR amplifications from oligo(dT) primed cDNA: (✔) A single correctly sized PCR amplicon was obtained; (—) no amplicon was obtained; (M and I) clones displaying Multiple PCR amplicons and a single incorrectly sized amplicon, respectively; (*) Amplification of a low yield correctly sized PCR amplicon. (Column 4) PCR-based chromosomal assignment of selected library clones using the Corriel somatic cell hybrid mapping panel (no. 2): (/) Clones for which primer pairs were rejected during prescreen assays; (×) clones for which primer pairs were rejected during chromosomal assignment assays; (H) high yield amplification of other chromosomal templates (hamster/mouse); (1*) highest yield amplicon from chromosome 1. (Column 5) Results of RH mapping of clones along their assigned chromosomes. The scale is centiRays (cR3000) with the zero at the p-telomere. (Column 6) Results of database searches.
