Figure 2.
Slot–blot hybridiation of DNA extracted from immunoprecipitated dinucleosomal chromatin fragments generated by MNase digestion of K562 nuclei. Two micrograms of sonicated genomic K562 DNA (Total), together with 2 μg of Input, Unbound, and antibody-Bound DNA were denatured and immobilized on nylon membranes. The slot–blot was probed with [α-32P]dCT-labeled c-myc, α-globin, and hGH gene sequences and visualized by autoradiography (A, B, and C, respectively). The ratio of Bound to Input hybridization signal (B/I) represents the enrichment achieved by the antibody.
