Figure 1.
(A) Ethidium bromide-stained 2% agarose gel of 10 μg DNA from sucrose gradient purified nucleosome oligomers in supernatants S1 and S2 generated by MNase digestion of K562 nuclei. Nuclei were digested at a concentration of 5 mg/ml using 300 units of MNase/mg DNA at 20°C for 2 min. (Lane 1) Sonicated genomic DNA; (lanes2,3) mono- and dinucleosomal DNA of S1, respectively; (lanes 4–7) mono-, di-, tri-, and tetranucleosomal DNA of S2, respectively. (B–D) Autoradiographs of Southern blot transfers of A hybridized with [α-32P]dCTP-labeled c-myc, α-globin, and hGH gene sequences, respectively. M denotes a DNA size marker.
