Chromosomal arms differentially painted by PQ-COBRA–FISH. PQ-COBRA–FISH to normal male metaphase chromosomes. (A) shows the superimposed, pseudocolor images of the three fluorophores used for ratio labeling the WCPs in a karyogram format. The first binary label (B) differentiates the two identically ratio-labeled WCP sets. The karyogram was generated automatically on the basis of chromosomal WCP–FISH ratios and the presence (odd numbered chromosomes plus Y) or absence (even numbered chromosomes plus X) of the first binary color. The second binary label, differentiating p and q arms, is displayed inC. All q arm paints were labeled with the second binary label, except for chromosomes 4 and 7, for which p arm paints were used. Note the disturbance of the COBRA–FISH signals on the X chromosome by the Y paint.
