
Quantitative allele frequency estimation based on two-color analysis of synthetic mixed templates. The two templates were mixed in the ratios of 1 nM/10 nM, 1 nM/3 nM, 1 nM/1 nM, 3 nM/1 nM, and 10 nM/1 nM, respectively. The logarithm of intensity ratios of the two colors (Y-axis) are plotted against the logarithm of concentration ratios of the two mixed templates (X-axis). FL, fluorescein intensity; PE, phycoerythrin intensity; G/T, concentration ratio of template G to template T.











