Identification of pericentromeric BAC clones. A total of 702 individual BAC clones were identified upon hybridization of the RPCI-11 BAC library (segments 1 and 2) with 101B6-derived probes. 107 of these clones were characterized at the sequence level with 16 of the paralogous STSs indicated by an underline. 65/107 BACs could be assigned to a chromosomal bin based on at least five diagnostic paralogous sequence variants between the BAC and monochromosomal hybrid signature. A representative subset of paralogous BACs are depicted.Filled circles show the representative STS content of each BAC based on amplification with 101B6-derived pSTSs. Open circlesindicate that a product larger than expected was amplified.Asterisks indicate BACs for which one (*) or both (**) end sequences were generated. Boxes show the position of the BAC-end sequence with respect to the 101B6 reference sequence. Eleven different contig bins were created corresponding to BACs from chromosome 1, 2, 4, 9, 10, 15, 16, 17, 22, acrocentric bin (13, 14, 15, 21, 22), as well as a miscellaneous bin, which includes BACs that have not yet been assigned to a chromosome but possess a distinct paralogous sequence signature.
