Identification and confirmation of the PnC DNA clone using the AT28 polymorphism. AT28 was amplified by PCR as described in Methods. PCR products were purified and digested with RsaI and electrophoresed on 3% (wt/vol) agarose. (BE) Cell line from mardel(10) patient; (BE2C1–18–5f) a somatic cell hybrid line containing mardel(10) chromosome but not the normal chromosome 10; (5f-52-E8) a BAC clone containing the NC region derived from the BE2C1–18–5f somatic cell hybrid; (CE-4–27) a circular YAC containing the PnC DNA; (CE) a cell line from patient BE's father; (PAC4) a PAC clone containing the HC DNA derived from the normal chromosome 10 of a CEPH individual (du Sart et al. 1997; Cancilla et al. 1998). Note the identical fingerprints of CE-4–27, BE2C1–18–5f and 5f-52-E8.
