PCR-based screening of BAC DNA pools for SSR markers. (A) BAC DNA plate pools (PP) and side pools (SP) were analyzed for the presence of two SSRs, Xtxp84 and Xtxp211. BAC DNA pools positive for a given SSR are identified above the respective lane. Plant genomic DNA (BTx623) was used as PCR template in control lanes labeled BTx. Fluorescent-labeled molecular weight standards (LI-COR) were loaded in the first and last lanes and their sizes (bp) are indicated to the right of the gels. (B) FPC V4.5 fingerprint analysis window displaying the representative fingerprint patterns of individual BAC clones from ctg190. Four BAC clones in ctg190 were identified as positive for Xtxp84, and three BACs were identified as positive for Xtxp211 (·) following analysis of the BAC DNA pools from A. (C) FPC V4.5 contig window displaying a horizontal representation of ctg190 that contains BAC clones positive for Xtxp84 and Xtxp211. The characters (*, = and ∼) to the right of individual clone names represent canonical, equal, and approximate clones, respectively (Soderlund et al. 1998). The shaded bar below the contig display indicates the length of the contig, measured in number of bands.
