
Autoradiograph of a typical 33P-labeled DNA fingerprint gel. Each lane represents the fingerprint analysis from an individual sorghum BAC clone digested with HindIII and HaeIII followed by labeling at the HindIII terminus. Labeled λ/Sau3A standard was loaded in the first and every ninth lane (λ). The asterisk marks the position of the common cloning vector band appearing in all BAC clone lanes. Arrows at the top and bottom of the image delineate the region of the gel that was used for band calling in Image 3.5.











