Construction of a BAC Contig Map of Chromosome 16q by Two-Dimensional Overgo Hybridization

Table 3.

Results of Two-Dimensional Hybridization

Source LANL Whitehead Stanford Total
STS–YAC STS–SCH RH map unmapped
No. of overgos 402 128 166 64 148 908
Column pools 24 24 24 24 24 120
Row pools 17 6 8 3 7 41
Overgos with at least 366 120 147 60 135 828
 one positive BAC (91%) (94%) (89%) (95%) (92%) (91%)
Overgos requiring 3 234 61 55 12 96 458
 hybridizations (64%) (51%) (37%) (20%) (71%) (50%)
Average hits per overgo 12.6 11.4 10.3 10.2 9 10.6
  • To resolve ambiguous BAC–probe relationships (see text for details).

This Article

  1. Genome Res. 10: 714-721

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