Construction of a BAC Contig Map of Chromosome 16q by Two-Dimensional Overgo Hybridization

Detailed contig map of 16q12.1–q12.2. The consensus restriction map is shown at top in black and cyan. The bold regions are covered by clones that are sequenced or being sequenced. The size and order of the fragments in these regions are confirmed or partially confirmed at the genomic sequence level. Only fragments that are >2 kb are listed. The markers are listed vertically under the restriction map. The lines connecting markers with the restriction map indicate that these markers have been found within the draft or finished sequence of the BACs. The markers in black are overgos that were screened by hybridization, those in magenta are STSs that were screened by PCR, and those in green are sequence identities identified by searching the BAC-end database with the sequences of the sequenced clones. The arrows in bold indicate the backbone markers from the previous LANL STS–YAC map. The BACs with names in red have been confirmed to map to 16q12.1–q12.2 by FISH. The BACs whose names sbegin with“R” are from the RPCI-11 library, and the rest are from the BAC library of the California Institute of Technology. (*FRAGMENT) Gap closed by restriction data only.