Figure 2.
Top pool hybridization. (A) Pool 1 with 96 overgos hybridized with the seventh high-density filter of the 12 RPCI-11 human BAC library. (B) Pool 3–4 with 192 overgos hybridized with the fourth high-density filter of the same BAC library. There are six panels on each filter and 4×4×384 plates gridded on each panel. Each plate is gridded in duplicate in different orientations on the membranes. The signals are graded according to their strength into four groups: strong, media, weak, and very weak. The signal in the first three group are picked up as positives.
