Null Mutation of PCLN-1/Claudin-16 Results in Bovine Chronic Interstitial Nephritis

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Figure 1.Figure 1.Figure 1.Figure 1.Figure 1.
Figure 1.

(a) Genetic (top) and physical (bottom) maps of the CINF critical region. The cosmid contig spans ∼100 kb. (E) EcoRI; (S) SacI; (X) XhoI. (b) Genotyping of DIK130. Sire and dam are offspring of grandsire. Both offspring are full siblings from sire and dam. The 123-bp allele of grandsire was not transmitted to sire and dam. (c) Southern blot of CINF-affected cattle. Genomic DNA from normal (lane 5), carrier (lanes 1,4) , and CINF-affected cattle (lanes 2,3) was digested withEcoRI. The blot was hybridized to a probe of 0.8 kb from the 5′ half of cDNA 55. Bar, 3.0-kb deleted DNA fragment. (d) Genomic structure of CL-16 and the deleted region.CL-16 is composed of five exons. The deleted region spans 37 kb and contains exons 1–4 of CL-16. In the mutant allele, there is an insertion at the deletion point. (e) Sequence of the deleted region. The G-rich inserted sequence is 31 bp.

This Article

  1. Genome Res. 10: 659-663

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