Genetic Linkage Mapping of Zebrafish Genes and ESTs

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Figure 1.
Figure 1.

Production of the homozygous diploid mapping panel. C32 and SJD fish were crossed to produce a heterozygous F1 generation. Eggs from two F1 females were fertilized with UV-irradiated sperm, which makes no genetic contribution to the progeny. The resulting haploid embryos were subjected to heat shock treatment, which doubles the haploid chromosome set. DNA for the mapping panel was prepared from homozygous diploid F2 adults.

This Article

  1. Genome Res. 10: 558-567

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