Table 3.
Primer Sequences
| Primer (exon) | Sequence (5′-3′) | Length | T m (°C) | Product (bp) | |||||||||||||||||||||
| 1, F | A | G | C | C | T | G | G | G | T | C | G | G | T | T | G | T | A | C | G | 19 | |||||
| 1, R | A | G | C | A | G | G | T | G | T | T | C | C | T | C | G | G | G | A | G | 19 | 60 | 684 | |||
| 2, F | A | T | T | G | T | G | T | C | T | T | C | A | T | G | A | C | T | T | C | 19 | |||||
| 2, R | G | A | A | T | T | C | T | T | C | C | T | C | A | T | T | C | A | C | 18 | 50 | 953 | ||||
| 3a, F | A | G | C | T | G | T | T | G | T | G | A | T | C | A | T | T | G | T | C | 19 | |||||
| 3a, R | G | A | G | T | T | G | T | G | G | T | G | G | T | T | G | A | A | G | 18 | 54 | 1122 | ||||
| 3b, F | T | C | C | C | A | C | G | A | C | T | A | C | C | A | C | C | A | A | G | 19 | |||||
| 3b, R | A | C | C | G | G | T | C | T | C | C | A | C | A | G | A | C | A | A | C | 19 | 55 | 1507 | |||
| 3c, F | G | C | C | G | G | C | A | C | C | T | C | C | A | G | T | T | T | A | T | C | C | 21 | |||
| 3c, R | G | T | C | A | T | G | T | T | C | T | T | G | G | G | A | C | G | G | G | A | G | C | 22 | 68 | 514 |
| 3d, F | C | C | T | T | T | A | C | G | G | C | C | C | C | C | A | T | G | G | A | G | 20 | ||||
| 3d, R | C | G | C | C | A | C | C | A | G | A | C | C | T | T | T | C | C | C | A | G | 20 | 65 | 1296 | ||
| 3e, F | T | C | C | C | A | A | C | A | G | T | C | C | A | C | A | G | C | C | C | 19 | |||||
| 3e, R | T | C | C | C | C | T | C | T | T | C | C | C | T | C | T | G | T | C | A | C | A | 21 | 61 | 1402 | |
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↵(F) Forward; (R) reverse.
