High-Throughput SNP Allele-Frequency Determination in Pooled DNA Samples by Kinetic PCR

Table 3.

Allele Frequency Measurements on a Pool of Mouse DNAs from 10 Different Inbred Strains

Determination TLR4 FASL AHR HOX2.3 REN1
 1 0.09 0.13 0.22 0.30 0.47
 2 0.10 0.14 0.20 0.28 0.43
 3 0.09 0.11 0.20 0.27 0.46
 4 0.10 0.13 0.20 0.28 0.48
 5 0.10 0.14 0.21 0.29 0.44
 6 0.10 0.16 0.18 0.27 0.45
 7 0.10 0.16 0.15 0.27 0.42
 8 0.09 0.13 0.20 0.29 0.42
 9 0.10 0.15 0.19 0.31 0.41
10 0.09 0.16 0.20 0.30 0.30
11 0.10 0.19 0.19 0.29 0.33
12 0.09 0.18 0.18 0.24 0.37
Measured  allele 1  frequency 0.10 0.15 0.19 0.28 0.42
Known  frequency 0.10 0.20 0.20 0.30 0.40
Standard  deviation (±) 0.005 0.023 0.018 0.017 0.054

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  1. Genome Res. 10: 258-266

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