High-Throughput SNP Allele-Frequency Determination in Pooled DNA Samples by Kinetic PCR

Table 2.

Allele Frequency Measurements on a Pool of 100 Human DNAs

Determination PON B71 CST5
 1 0.45 0.72 0.41
 2 0.49 0.75 0.37
 3 0.41 0.72 0.39
 4 0.43 0.72 0.43
 5 0.46 0.74 0.41
 6 0.45 0.73 0.42
 7 0.44 0.72 0.42
 8 0.47 0.73 0.42
 9 0.49 0.76 0.42
10 0.44 0.81 0.40
11 0.44 0.71 0.42
12 0.44 0.74 0.41
Measured  allele 1  frequency 0.45 0.74 0.41
Known  frequency 0.43 0.75 0.44
Standard  deviation (±) 0.024 0.027 0.018

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  1. Genome Res. 10: 258-266

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