High-Throughput SNP Allele-Frequency Determination in Pooled DNA Samples by Kinetic PCR

Table 1.

Allele Frequency Measurements Across a Range of Values

Allele 1 frequency (by OD260) Expected ΔCt PON B71
average observed ΔCt heterozygote corrected ΔCt measured allele 1 frequency standard deviation (±) average observed ΔCt heterozygote corrected ΔCt measured allele 1 frequency standard deviation (±)
0.95 −4.25 −4.01 −4.05 0.94 0.016 −3.21 −3.77 0.93 0.012
0.90 −3.17 −2.99 −3.03 0.89 0.010 −2.23 −2.79 0.87 0.023
0.80 −2.00 −1.88 −1.92 0.79 0.014 −1.22 −1.78 0.77 0.040
0.67 −1.00 −1.02 −1.06 0.68 0.051 −0.21 −0.77 0.63 0.032
0.59 −0.50 −0.44 −0.48 0.58 0.030 0.24 −0.32 0.56 0.031
0.50 0.00 0.14 0.10 0.48 0.016 0.70 0.14 0.48 0.024
Het. 0.00 0.04 0.00 0.50 0.051 0.56 0.00 0.50 0.013
0.42 0.50 0.78 0.74 0.37 0.045 1.21 0.66 0.39 0.016
0.33 1.00 1.18 1.14 0.31 0.007 1.65 1.09 0.32 0.021
0.20 2.00 2.11 2.07 0.19 0.007 2.54 1.99 0.20 0.011
0.10 3.17 3.21 3.17 0.10 0.014 3.63 3.07 0.11 0.007
0.05 4.25 4.52 4.48 0.04 0.003 4.68 4.12 0.05 0.002

  • Ct measurements are average of four replicates.

This Article

  1. Genome Res. 10: 258-266

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