Table 2.
PCR Primers Prepared for Evaluation of Expressed Sequences and Their Location to the Physical Map
| Sequence name | Primer | Ta (C) | Prod size (bp) | YAC testing | Contig testing | Source | |||||||
| forward 5′ → 3′ | reverse 5′ → 3′ | ||||||||||||
| D78212 | TCCCAAAACAGAAAGAGCAGATGT | GTGGGGCAGGTGTTCCAATAAA | 50 | 121 | 932 e 9 | − | Chinen et al (1996) | ||||||
| D78215 | CCACCGGCAGGAATGAACAAC | CGCTCTAGTGCCGTCTGAGTCTGT | 60 | 165 | − | − | Chinen et al (1996) | ||||||
| D78216 | ATCAGTACAAGGGGATTCTAACT | ATGTTTTCTTAACGAGGAGACA | 50 | 82 | − | − | Chinen et al (1996) | ||||||
| D78221 | CCCCTCAGGATTGACTAT | TCACGTCACCTTTTGCAT | 50 | 104 | − | − | Chinen et al (1996) | ||||||
| D78225 | ACAGCCCCTCAGGATTGACTA | GAGGCATCATCGAAGGTAAGG | 55 | 88 | − | − | Chinen et al (1996) | ||||||
| D78226 | AGAAGCCCCGAAGAGACACCAA | GGCGCTTTGCCAGGGAAGA | 55 | 134 | − | − | Chinen et al (1996) | ||||||
| D78227 | GCACCGTTAACAACATGTCTATTT | GAAGAGCCAAGGAAGAGAAACTT | 50 | 143 | − | − | Chinen et al (1996) | ||||||
| D78228 | TTCAGGTCATTTTTCCAGTTT | CTTCCCTATAGAACGACTTG | 50 | 90 | − | − | Chinen et al (1996) | ||||||
| D78229 | ATGCGCTACCCCCTGAGAAAAC | GCAGGTGCTGAATCACAACTGTCA | 55 | 147 | − | − | Chinen et al (1996) | ||||||
| D78230 | TTTTTGAGCTAGAGTTCACATAGA | TGGCAAGCGAATTCACTTCT | 50 | 85 | − | − | Chinen et al (1996) | ||||||
| D78231 | AGAGGAAAGGCCACATGAGGACA | AGTGTTGGCAGGTCTGATTTCTCC | 60 | 90 | − | − | Chinen et al (1996) | ||||||
| D78233 | CCTAGGACCCCTGCCTCAG | CCAAAGGGTGGTCAAAGTAGGT | 60 | 81 | − | − | Chinen et al (1996) | ||||||
| D78234 | TCCTGGCCTAGAGGTCTTA | TGTTTTTGTAGGTCTCAATCTTA | 50 | 103 | − | − | Chinen et al (1996) | ||||||
| D78237 | TATCTTGAGCCAACCTCCTGTCTC | TCTTGATAGGGGCTGGGTAAAATA | 55 | 103 | 932 e 9 | B-222N15, B-222I10 | Chinen et al (1996) | ||||||
| D78239 | CTATCCATGCTAAGACATCC | TGTATAAAGGAAAGAAGTTGAATG | 50 | 98 | − | − | Chinen et al (1996) | ||||||
| D78242 | CGGCCTATGCGACGAGTCA | GTAATTTCGCTGCTTCCAGTCG | 50 | 111 | 932 e 9 | − | Chinen et al (1996) | ||||||
| D78246 | TTCTGCTTTTCCTGGACCTGTTCC | CACCGGGCTGGGAGGACTGT | 55 | 123 | − | − | Chinen et al (1996) | ||||||
| D78247 | CCCCAGTTGAGCCATGAGATAA | TTGGCAATTTCTGTAAGTCAGGAA | 55 | 123 | − | − | Chinen et al (1996) | ||||||
| R80652 | GTCTGATGTCACTCAGATTGAAGG | TGAGCAAAAAGTCTATGCCTAAA | 55 | 109 | 932 e 9 | B-350A20 | Chinen et al (1996) | ||||||
| WI-17604 | TGGGCCAAGAGCTTTCTG | TAGACTACTTTGGAGATGCAGCC | 50 | 139 | 932 e 9 | B-350A20 | Hum. Gene Map, RHdb | ||||||
| 20B08e1 | CAATGAGAGGGGCTTTGT | GTTTCCATGGGATGTTCA | 50 | 70 | 932 e 9 | P-20B08 | exon amplification | ||||||
| 20B08e4 | ACTGGCCACCACCAGCGTGAT | GAGCAAGATGGCAGGGGTGGTGTG | 55 | 102 | 932 e 9 | P-20B08 | exon amplification | ||||||
| 20B08e5 | GGGAATTTTAGGTGGTGGTGGTTC | AGGATTCAGGCATAATGGTTTAGC | 55 | 191 | 932 e 9 | P-20B08 | exon amplification | ||||||
| R26196 | GAATCGTTCCATCATATCCACA | TGACAGCAGGTAACCGATACC | 55 | 201 | 932 e 9 | B-33B22, P-20B08 | RHdb | ||||||
