Table 2.

Comparison of Chargaff Differences of Six Segments ofM. jannaschii

Genome segment		Chargaff difference (%)		Base composition of average 1-kb window		Purine-loading index
no.		(C + G) %		number of windows						transcription orientation		leftward-transcribed		rightward-transcribed
no.		(C + G) %		leftward	rightward					leftward	rightward	A	C	G	T	A	C	G	T
1	32.1	1370	1169	(A − T)/W	−10.66 ± 0.21	9.93 ± 0.28	303	203	118	376	373	124	197	306	298
				(C − G)/S	26.48 ± 0.33	−23.04 ± 0.40	A − T = −73		C − G = 85		A − T = 67		C − G = −73
2	31.6	1416	1257	(A − T)/W	−8.38 ± 0.22	8.46 ± 0.27	313	193	123	371	371	123	193	313	256
				(C − G)/S	21.95 ± 0.43	−22.37 ± 0.37	A − T = −58		C − G = 70		A − T = 58		C − G = −70
3	31.7	1305	1324	(A − T)/W	−9.92 ± 0.21	9.65 ± 0.20	308	197	120	375	374	121	196	309	284
				(C − G)/S	24.45 ± 0.30	−23.69 ± 0.35	A − T = −67		C − G = 77		A − T = 65		C − G = −75
4	30.9	1248	1368	(A − T)/W	−9.82 ± 0.23	10.40 ± 0.21	312	191	118	379	381	113	196	310	294
				(C − G)/S	23.34 ± 0.35	−26.64 ± 0.32	A − T = −67		C − G = 73		A − T = 71		C − G = −83
5	31.5	1180	1361	(A − T)/W	−8.80 ± 0.27	10.57 ± 0.20	312	195	120	373	379	123	192	306	278
				(C − G)/S	23.98 ± 0.33	−22.08 ± 0.35	A − T = −61		C − G = 75		A − T = 73		C − G = −69
6	30.9	975	1723	(A − T)/W	−11.48 ± 0.23	10.31 ± 0.18	306	192	117	385	381	114	195	310	306
				(C − G)/S	24.23 ± 0.40	−26.24 ± 0.30	A − T = −79		C − G = 75		A − T = 71		C − G = −81

Details are as in Table 1.
↵The six segments correspond to bases 1–276500, 276501–553000; 553001–829500, 829501–1106000, 1106001–1382500, and 1382501–1664970.

Thermophilic Bacteria Strictly Obey Szybalski's Transcription Direction Rule and Politely Purine-Load RNAs with Both Adenine and Guanine