Detection of Deleted Genomic DNA Using a Semiautomated Computational Analysis of GeneChip Data

Table 1.

Deletion Screen of CDC1551 Shows that TSTEP/Deletion Assembly Finds All Deletions >350 bp in Nonrepetitive Regions, with No False Deletion Cells

H37Rv regions of at least 300 bp with no identical match in CDC1551 (to the nearest 60 bp) Repetitive sequence family in probe set annotation Similarity with any region by blast to CDC1551 (%) Edges detectable by blast to CDC1551 (corresponding address in H37Rv is given) Deletion in nonrepetitive region, with detectable edges >350 bp Complete TSTEP/deletion assembly results for screen of CDC1551
Start Stop Length Start Stop Length Start Stop Length
333540 336300 2760 PE/PGRS 99.3 No
336360 339000 2640 PE/PGRS 97.1 No
835920 841080 5160 PE/PGRS 99.6 No
886500 887460 960 886541 887414 873 Yes 886427 887553 1127
1217460 1218180 720 PE/PGRS 72.9 No
2045040 2045400 360 PE/PGRS 95.0 No
2060280 2060700 420 87.4 No
2339220 2339640 420 2339258 2339503 245 No
2381400 2383740 2340 Overlaps PPE 2381413 2383683 2270 Yes 2380893 2383514 2622
2704260 2704920 660 2704308 2704805 497 Yes 2704242 2704695 454
3054660 3054960 300 PE/PGRS 3054720 3054906 186 No
3122580 3123300 720 3122828 3123145 517 Yes 3122606 3123248 643
3501300 3501720 420 PPE 3501331 3501662 331 No
3730860 3732180 1320 PPE 3730999 3732075 1076 No
3732600 3735720 3120 PPE 3732653 3735632 2979 No
3842280 3846600 4320 Overlaps PPE 3842305 3846512 4207 Yes 3842882 3846825 3944
3894600 3894900 300 PPE 91.3 No
3895020 3895320 300 PPE 86.4 No
3933480 3934020 540 PE/PGRS 96.7 No
3934080 3936660 2580 PE/PGRS 89.9 No
3939720 3945120 5400 PE/PGRS 99.0 No
3946860 3950340 3480 PE/PGRS 69.2 No
3955440 3956100 660 3955464 3956100 636 Yes 3955252 3956433 1182
  • (TSTEP) Tandem Set Terminal Extreme Probability.

  • More than one region with 60% or more identity is found in this interval. A weighted average is reported.

  • There are only three probe pairs querying the 245-bp interval. A weak signal of P value <0.01 is measured for these probe pairs.

  • PPE locus at 2381069–2382490.

  • PPE loci at 3842235–3842765 and 3843032–3843730.

This Article

  1. Genome Res. 10: 2044-2054

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