Gene Discovery Using Computational and Microarray Analysis of Transcription in the Drosophila melanogaster Testis

Table 1.

Testis cDNA Library Characterization

Testis cDNA library
unamplified 8 × 106pfu
amplified 1.8 × 1012 pfu
mass excised 4.7 × 1013 cfu
Insert characterization
size selection 1–7 kb
inserts >1 kb 74% (n = 173)
no insert <<1% (n = 3,408)
chimeric 5% (n = 86)
initiating AUG present 67% (n = 139)
longer (5′) than BDGP EST 39% (n = 385)

  • ↵Includes testis, vas deferens, and portions of ejaculatory apparatus and accessory glands.

  • ↵n = number tested for indicated property.

  • ↵Clones where: BLAST E-values versus nr or BDGP EST databases are <1E-20, orientation was plus/plus, and identity = 100%.

This Article

  1. Genome Res. 10: 2030-2043

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