Table 1.
Comparison of Results of Mouse Brain and Human Transcriptome
| Copies/cell | Mouse brain transcriptome | Human transcriptome (Velculescu et al. 1999) | |||||
| N (% of total) | % of cellular transcripts | N (% of total) | % of cellular transcripts | ||||
| >500 | Unique TAGs | 33 (0.07%) | 11% | 55 (0.04%) | 18% | ||
| GenBank matches | 26 (78%) | 55 (100%) | |||||
| 50–500 | Unique TAGs | 709 (1.55%) | 26% | 578 (0.43%) | 27% | ||
| GenBank matches | 587 (82%) | 576 (99%) | |||||
| 50–50 | Unique TAGs | 7,821 (17%) | 34% | 6,160 (4.6%) | 30% | ||
| GenBank matches | 3,548 (48%) | 5,913 (96%) | |||||
| ≤5 | Unique TAGs | 37,293 (81%) | 28% | 127,342 (94.9%) | 25% | ||
| GenBank matches | 6,927 (18%) | 66,091 (52%) | |||||
| Total collected TAGs | 45,856 | 100% | 134,135 | 100% | |||
| GenBank matches | 11,088 (24%) | 72,635 (54%) | |||||
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Tags were distributed according to their copy number per cell into four categories: >500 copies, 50–500 copies, 5–50 copies, and ≤5 copies per cell (on estimate of 300,000 total transcripts per “average cell” was used). The number of unique transcripts/tags and the corresponding GenBank matches are shown for each category. The percentage of total cellular transcripts contributed by each category is also shown.
