Representative alignment of Sequences of Known Serpins.
Regions of secondary structure seen in 1OVA, 2PSI and 1AZX are displayed; cylinders represent helices and arrows represent sheets. The variability (Wu & Kabat 1970) is shown by the jagged line above the sequences. Sequence numbering is according to α1–antitrypsin. Residues are colored according to strict conservation (across all 219 serpin sequences): The darker the shading, the more highly conserved. The following graduations are used: 0–20% (white), 20%–30%, 30%–40%, 40%–50%, 50%–60% and 60%–70%. Residues conserved in >70% of sequences are in dark red and are listed in Table 5. Species abbreviations: ath, Arabidopsis thaliana; bma, Brugia malayi; bmo, Bombyx mori; dme, Drosophila melanogaster; gga, Gallus gallus; hsa, Homo sapiens; hvu, Hordeum vulgare; mvi, Myxoma virus; oar, Ovis aries; pma, Petromyzon marinus; sma, Schistosoma mansoni; svi, Swinepox virus; ttr, Tachypleus tridentatus; tae, Triticum aestivum; vavi, Variola virus. Serpin name abbreviations: A2AP, α2–antiplasmin; AAT, α1–antiproteinase inhibitor or α1–antitrypsin; ACT, antichymotrypsin; ANGT, angiotensinogen; ANT, antithrombin; C1-I, C1 inhibitor; CBG, cortisol-binding globulin; GDN, glia derived nexin or poteinase nexin-1; HEPII, Heparin Cofactor II; HSP47, 47 kDa heat shock protein; KAL, kallistatin; LICI, limulus intracellular coagulation inhibitor; MNEI, monocyte/neutrophil elastase inhibitor; NEUS, neuroserpin; OVAL, ovalbumin; PAI-1, PAI-2 etc., Plasminogen Activator Inhibitor-1 -2 etc; PCI, protein C inhibitor; PEDF, pigment epithelium derived factor; PI-6, PI-8, PI-9 etc., proteinase inhibitor; Put, putative; SCCA, squamous cell carcinoma antigen; SERP, serpin; SPI-1, SPI-2 etc., serine proteinase inhibitor; THBG, thyroxine binding globulin; UTMP, uterine milk protein.
